Homeobox Genes DataBase






Drosophila/Vertebrate Genes in Development:








Segment polarity genes











engrailed






Definition:

en is expressed in fourteen evenly spaced domains that become the
anterior part of each parasegment. engrailed expression has been
targeted to different regions of the wing disc.


Function:

The engrailed gene helps to direct Drosophila melanogaster development
by encoding a homeodomain-containing DNA binding protein. (Saenz-Robles
M.T., 1995)


Protein:

Homeodomain-containing DNA binding protein.






Expression patterns:

Early Segmentation

Wing Imaginal Discs


en expression is diminished in wg and arm mutants. An allelic series
was described for each with with increasingly severe segment polarity phenotypes
and earlier and more severe loss of en expression. The segmental stripes
decay with a similar asymmetric pattern in wg and arm mutants. (Peifer
M., 1991)


en expression was observed sequentially in 5 'centers' anterior to the
mandibular segment starting at embryonic stage 8.(Schmidt-Ott
U., 1992)


The en protein is expressed in a specified subset of neuroblasts in
embryonic stages 8-11. (Doe C.Q., 1992)


Delayed changes in en expression were observed in response to ectopic
eve expression in eve[hs.PS] embryos. Four different expression patterns
were observed depending on the timing of ectopic eve protein induction.
(Manoukian A.S., 1992)






Regulatory Regions:








Regulatory element

Localisation of element

Trans-regulatory factor

References





engrailed 5'-flank

promoter region

HUNCHBACK


KRUPPEL


Zuo et al., 1991









Regulatory Connections




Regulatory Connections:




Downstream Genes:






Upstream Genes:








Sequences:

1. [U58775] Heliocidaris erythrogramma engrailed homeobox protein (HeEn) gene,
3' exon, partial cds.

2. [U41757] Mus musculus engrailed-2 gene, 5' regulatory sequence.

3. [W30468] mc20f11.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 349101
5' similar to gb:L12703 Mouse engrailed protein (MOUSE);.

4. [W30468] mc20f11.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 349101
5' similar to gb:L12703 Mouse engrailed protein (MOUSE);.

5. [X70939] A.franciscana mRNA for engrailed homeobox protein.

6. [M64335] Bombyx engrailed (Bm en) mRNA, complete cds.

7. [U21120] Beroe ovata engrailed homeobox protein (Cteno-en) gene, partial
cds.

8. [U23153] Cadulus fusiformis engrailed homolog gene, partial cds.

9. [U26639] Ctenodrilus serratus engrailed class homeobox (CTs-en) mRNA, partial
cds.

10. [U23214] Crassostrea virginica engrailed homolog gene, partial cds.

11. [U23154] Dentalium eboreum engrailed homolog gene, partial cds.

12. [M29285] D.melanogaster engrailed (en) gene encoding a developmental protein,
5' flank.

13. [K03055] D. melanogaster engrailed locus, engrailed gene, exon 2.

14. [K03056] D. melanogaster engrailed locus, engrailed gene, exon 3.

15. [K03057] D. melanogaster engrailed locus, engrailed-related gene, exon
3.

16. [K03058] D. melanogaster engrailed locus, engrailed-related gene, exon
2.

17. [M29286] D.virilis engrailed (en) gene encoding a developmental protein,
5' flank.

18. [K03059] Drosophila melanogaster engrailed locus, engrailed gene, exons
2 and 3.

19. [M10017] Drosophila melanogaster engrailed protein (en) mRNA, complete
cds.

20. [X04727] Drosophila virilis en-gene (engrailed gene) involved in segmentation.

21. [X58692] H. triserialis DNA homologous to the Drosophila engrailed gene.

22. [U23432] Ilyanassa obsoleta engrailed homolog gene, clone A, partial cds.

23. [U23433] Ilyanassa obsoleta engrailed homolog gene, clone B, partial cds.

24. [U21675] Lepidochitona caverna engrailed homolog gene, partial cds.

25. [U21857] Nautilus pompilius engrailed homolog gene, partial cds.

26. [U23431] Nautilus pompilius engrailed homolog gene, clone A, partial cds.

27. [U23213] Placopecten magellanicus engrailed homolog gene, partial cds.

28. [S73225] engrailed homolog [Tribolium castaneum=red flour beetles, Genomic/mRNA,
1822 nt].

29. [S81027] Msr-110=EN protein binding gene/engrailed nuclear homeoprotein-regulated
gene [Drosophila melanogaster, mRNA, 2614 nt].

30. [M29262] Grasshopper engrailed (G-en) mRNA encoding a developmental protein,
partial cds.

31. [M85305] Schistosoma mansoni engrailed-like homeodomain protein (smox-2)
mRNA, complete cds.

32. [M19709] T.gratilla engrailed class homeo box gene, partial cds.

33. [X62688] T.retusa DNA for brachiopod engrailed homeodomain.

34. [U23212] Transennella tantilla engrailed homolog gene, partial cds.

35. [L12698] Human engrailed protein (EN1) gene, 5' end.

36. [L12699] Human engrailed protein (EN1) gene, 3' end.

37. [L12700] Human engrailed protein (EN2) gene, 5' end.

38. [L12701] Human engrailed protein (EN2) gene, 3' end.

39. [U41757] Mus musculus engrailed-2 gene, 5' regulatory sequence.

40. [L12702] Mouse engrailed protein (En-1) gene, 5' end.

41. [L12703] Mouse engrailed protein (En-1) mRNA, complete cds.

42. [L12704] Mouse engrailed protein (En-2) gene, 5' end.

43. [L12705] Mouse engrailed protein (En-2) mRNA, complete cds.

44. [X59125] B.rerio partial engrailed-related homeobox gene En-1.

45. [X59126] B.rerio partial engrailed-related homeobox gene En-2.

46. [X68151] B.rerio eng-2 (engrailed-2) gene.

47. [X07622] Zebrafish engrailed-like homeobox sequence.

48. [L12694] Gallus gallus engrailed protein (En-1) gene, 5' end.

49. [L12695] Gallus gallus engrailed protein (En-1) gene, 3' end.

50. [L12696] Gallus gallus engrailed protein (En-2) gene, 5' end.

51. [L12697] Gallus gallus engrailed protein (En-2) gene, 3' end.

52. [X59122] L.planeri partial engrailed related homeobox gene (En).

53. [X59120] M.glutinosa engrailed-related gene A (En-A) homeobox (partial).

54. [X59121] M.glutinosa engrailed-related gene B (En-B) homeobox (partial).

55. [X59123] X.laevis partial engrailed-related homeobox gene 1a (En-1a).

56. [X59124] X.laevis partial engrailed-related homeobox gene 1b (En-1b). 57.





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  • 
Zecca, M.. Basler, K., and Struhl, G. (1995). Sequential organizing activities
of engrailed, hedgehog and decapentaplegic in the Drosophila
wing. Development 121: 2265-2278 

    


    
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